INTERNATIONAL BURCH UNIVERSITY
Graduate Study - Faculty of Engineering and Natural Sciences
Genetics and Bioengineering PhD
2012-2013

SYLLABUS
Code Name Level Year Semester
GBE 622 Forensic DNA Technology Graduate 1 Fall
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
7.5 0 English

Instructor Assistant Coordinator
Damir Marjanović, Prof. Dr. Damir Marjanović, Prof. Dr.
[email protected] no email

COURSE OBJECTIVE
Concept of genetic polymorphism.
Scientific background of DNA analysis in legal medicine and forensic genetics. Contribution of forensic DNA analysis to juridical science development.
Genomic (nuclear) DNA and mitochondrial DNA in forensic genetics. Analysis of X and Y chromosome.
Accurate sampling for DNA analysis. DNA isolation from blood, hair root and bone. DNA isolation from cigarette end, glass swab, blood trace or sperm on clothes.
Methods of DNA analysis in forensic genetics: RFLP, PCR, STR’s analysis. Basic forensic DNA laboratory procedures. CODIS, INTERPOL, European data base.
Determining gender. Determining identity. Identification of war casualties using DNA analysis. Paternity testing. Interpretation of DNA analysis results.
Usage of DNA analysis in identifying crime perpetrator.
Possibility of DNA profiling. Possibility of testing disputable paternity. Making criteria for future education in forensic genetics.
The newest technologies and its usage in the field of FG.

COURSE CONTENT
Week
Topic
  1. Presentation of Syllabus and Course. Introduction with the literature and student project topics.
  2. Introduction to forensic genetics: Scientific background of DNA analysis in legal medicine and forensic genetics. Contribution of forensic DNA analysis to juridical science development
  3. Basic concept of genetic polymorphism in forensic genetics.
  4. Mitochondrial DNA in forensic genetics. Analysis of X and Y chromosome.
  5. Description of basic CSI procedure. Accurate sampling for DNA analysis. DNA isolation from blood, hair root and bone. DNA isolation from cigarette end, glass swab, blood trace or sperm on clothes.
  6. Inside the laboratory: Basic forensic DNA laboratory procedures – DNA isolation, DNA quantification, PCR, DNA profiling
  7. Statistical approaches in Forensic Genetics
  8. Paternity testing, DVI identification.
  9. Non-human forensic Genetics

  1. Forensic DNA data-bases and legislatives
  2. In the court room, Court testimony, Presentation of the Cases.
  3. The newest technologies: future developments within field of forensic genetics
  4. Analysis of skeletal remains from different periods. Forensic genetics and bio-terrorism
  5. Presentation of the Projects

LABORATORY/PRACTICE PLAN
Week
Topic

    TEACHING/ASSESSMENT
    Description
    • Lectures
    • Presentation
    • Seminar
    • Project
    • Case Studies
    • Demonstration
    Description (%)
    Method Quantity Percentage (%)
    Homework10
    Project20
    Midterm Exam(s)20
    Presentation10
    Final Exam140
    Total: 100
    Learning outcomes
    • usage all sorts of forensic and genetic analysis methods in processing human, animal and plant biological trace samples
    • collecting forensic samples for DNA analysis
    • getting experience in all sorts of theoretical knowledge in forensic science based on newest methods of DNA technology and its usage in legal medicine and juridical procedures.
    • identification based on low-copy DNA samples commonly used for identification of crime perpetrators, mass disaster casualties and paternity testing and understanding techniques currently used in forensic genetics including short tandem repeats (STR) analysis and interpretation, population genetics and mitochondrial and Y chromosome markers.
    • understanding basic techniques important for creating and usage of National DNA data base and understanding basic biostatistical principles in forensic genetics and introduction of the newest molecular biology techniques used in forensic sciences - understanding of problems in applied forensic genetics and studying reference literature of this field
    TEXTBOOK(S)
    • Butler JM. Forensic DNK Typing: Methodology, Technology and Genetics of STR Markers (2nd edition). London: Elsevier Academic Press; 2012.
    • Gunn A. Essential Forensic Biology (2nd edition). Chichester: John Willey & Sons;2009.
    • Houck MM, Siegel JA. Fundamentals of Forensic Science. London: Elsevier Academic Press; 2006.
    • Various scientific papers from the journals: Forensic Science International: Genetics and Croatian medical Journal

    ECTS (Allocated based on student) WORKLOAD
    Activities Quantity Duration (Hour) Total Work Load
    Lecture (14 weeks x Lecture hours per week)480
    Laboratory / Practice (14 weeks x Laboratory/Practice hours per week) 0
    Midterm Examination (1 week) 0
    Final Examination(1 week) 0
    Preparation for Midterm Examination 0
    Preparation for Final Examination7.50
    Total Workload: 0
    ECTS Credit (Total workload/25): 0