INTERNATIONAL BURCH UNIVERSITY
Graduate Study - Faculty of Engineering and Natural Sciences
3+2 Genetics and Bioengineering
2017-2018

SYLLABUS
Code Name Level Year Semester
GBE 523 Techniques in Molecular Biology Graduate 1 Spring
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
6 150

Instructor Assistant Coordinator
Lejla Smajlović Skenderagić, Assist. Prof. Dr. Assist. Prof. Dr. Lejla Smajlovic Skenderagic Lejla Smajlović Skenderagić, Assist. Prof. Dr.
[email protected] [email protected] no email

This course is organized as an advanced-level explanation of molecular biology techniques for professionals in genetics and bioengineering. Apart from basic techniques, such as DNA, RNA, and protein isolation, spectrophotometry, electrophoresis, and PCR, advanced topics in molecular biology are introduced as well. Some of those topics are: primer design, two-dimensional electrophoresis, blotting techniques, in situ hybridization, etc. Occasional practical sessions are organized in order to remind graduates on the basic postulates of laboratory work in molecular biology.

COURSE OBJECTIVE
The cognitive, affective and behavioral objectives of this course are following:
- Illustrating different methods of isolation of nucleic acids and proteins.
- Revising the advantages and drawbacks of different quantification methods.
- Enabling graduates to perform analysis of different biomolecules practically.
- Introduction to primer design.
- Demonstrating how to prepare radioactively-labeled nucleic acid probes and how to perform blotting techniques.
- Enabling graduates to perform bacterial transformation in a safe environment.
- Introduction to NGS.

COURSE CONTENT
Week
Topic
  1. Introduction to the course
  2. DNA and RNA isolation methods
  3. Protein isolation methods
  4. UV/vis spectrophotometry
  5. Vertical and horizontal electrophoresis
  6. Two-dimensional electrophoresis
  7. Thermal cycling (PCR and qPCR)
  8. MID-TERM EXAM
  9. Primer design
  10. Preparation of radiolabeled DNA and RNA probes
  11. Southern, Northern, and Western blotting
  12. In situ hybridization
  13. Restriction digestion and ligation of DNA fragments
  14. Bacterial transformation: Plasmid isolation, vectors, and plasmid insertion
  15. Next-generation / Massive parallel sequencing (NGS /MPS)

LABORATORY/PRACTICE PLAN
Week
Topic

    TEACHING/ASSESSMENT
    Description
    • Interactive Lectures
    • Presentation
    • Discussions and group work
    • Problem solving
    Description (%)
    Method Quantity Percentage (%)
    Midterm Exam(s)140
    Final Exam140
    +Seminar & Assignment 1 20
    Total: 100
    Learning outcomes
    • Students should be able to use different isolation methods of nucleic acids and proteins.
    • Students should be able to conduct several types of quantification methods.
    • Students should be able to create primers and perform PCR.
    • Students should be able to prepare radioactively-labeled nucleic acid probes
    • Students should be able to perform different blotting techniques
    • Students should be able to conduct bacterial transformation in a safe environment
    • Students should be able to recall the basic terms about NGS/MPS.
    TEXTBOOK(S)
    • Wilson, K., & Walker, J. (Eds.). (2010). Principles and techniques of biochemistry and molecular biology. Cambridge: Cambridge university press.
    • Walt Ream & Katharine G. Field (1998). Molecular Biology Techniques : An Intensive Laboratory Course, Elsevier Science Publishing Co Inc

    ECTS (Allocated based on student) WORKLOAD
    Activities Quantity Duration (Hour) Total Work Load
    Lecture (14 weeks x Lecture hours per week)15345
    Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)15230
    Midterm Examination (1 week)122
    Final Examination(1 week)122
    Preparation for Midterm Examination11010
    Preparation for Final Examination12020
    Assignment / Homework/ Project12121
    Seminar / Presentation12020
    Total Workload: 150
    ECTS Credit (Total workload/25): 6