INTERNATIONAL BURCH UNIVERSITY
Graduate Study - Faculty of Engineering and Natural Sciences
3+2 Genetics and Bioengineering
||Techniques in Molecular Biology
||Number of ECTS Credits
||Class Hours Per Week
||Total Hours Per Semester
|Lejla Smajlović Skenderagić, Assist. Prof. Dr.
||Assist. Prof. Dr. Lejla Smajlovic Skenderagic
||Lejla Smajlović Skenderagić, Assist. Prof. Dr.
|This course is organized as an advanced-level explanation of molecular biology techniques for professionals in genetics and bioengineering. Apart from basic techniques, such as DNA, RNA, and protein isolation, spectrophotometry, electrophoresis, and PCR, advanced topics in molecular biology are introduced as well. Some of those topics are: primer design, two-dimensional electrophoresis, blotting techniques, in situ hybridization, etc. Occasional practical sessions are organized in order to remind graduates on the basic postulates of laboratory work in molecular biology.
|The cognitive, affective and behavioral objectives of this course are following:
- Illustrating different methods of isolation of nucleic acids and proteins.
- Revising the advantages and drawbacks of different quantification methods.
- Enabling graduates to perform analysis of different biomolecules practically.
- Introduction to primer design.
- Demonstrating how to prepare radioactively-labeled nucleic acid probes and how to perform blotting techniques.
- Enabling graduates to perform bacterial transformation in a safe environment.
- Introduction to NGS.
- Introduction to the course
- DNA and RNA isolation methods
- Protein isolation methods
- UV/vis spectrophotometry
- Vertical and horizontal electrophoresis
- Two-dimensional electrophoresis
- Thermal cycling (PCR and qPCR)
- MID-TERM EXAM
- Primer design
- Preparation of radiolabeled DNA and RNA probes
- Southern, Northern, and Western blotting
- In situ hybridization
- Restriction digestion and ligation of DNA fragments
- Bacterial transformation: Plasmid isolation, vectors, and plasmid insertion
- Next-generation / Massive parallel sequencing (NGS /MPS)
- Interactive Lectures
- Discussions and group work
- Problem solving
| Final Exam||1||40|
| +Seminar & Assignment ||1 ||20|
- Students should be able to use different isolation methods of nucleic acids and proteins.
- Students should be able to conduct several types of quantification methods.
- Students should be able to create primers and perform PCR.
- Students should be able to prepare radioactively-labeled nucleic acid probes
- Students should be able to perform different blotting techniques
- Students should be able to conduct bacterial transformation in a safe environment
- Students should be able to recall the basic terms about NGS/MPS.
- Wilson, K., & Walker, J. (Eds.). (2010). Principles and techniques of biochemistry and molecular biology. Cambridge: Cambridge university press.
- Walt Ream & Katharine G. Field (1998). Molecular Biology Techniques : An Intensive Laboratory Course, Elsevier Science Publishing Co Inc
|ECTS (Allocated based on student) WORKLOAD
|Lecture (14 weeks x Lecture hours per week)||15||3||45|
|Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)||15||2||30|
|Midterm Examination (1 week)||1||2||2|
|Final Examination(1 week)||1||2||2|
|Preparation for Midterm Examination||1||10||10|
|Preparation for Final Examination||1||20||20|
|Assignment / Homework/ Project||1||21||21|
|Seminar / Presentation||1||20||20|