INTERNATIONAL BURCH UNIVERSITY
Graduate Study - Faculty of Engineering and Natural Sciences
Genetics and Bioengineering Master With Thesis
2016-2017

SYLLABUS
Code Name Level Year Semester
GBE 512 Microbial Genetics Graduate 1 Fall
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
Area Elective 6 3 150 English

Instructor Assistant Coordinator
Mirsada Hukić, Prof. Dr. Prof.Dr. Mirsada Hukić Mirsada Hukić, Prof. Dr.
[email protected] [email protected] no email

This course focuses on how bacteria and bacteriophages arrange and rearrange their genetic material through mutation, evolution, and genetic exchange to take optimal advantage of their environment. The course is divided into three sections, namely DNA metabolism, genetic response, and genetic exchange. The first part addresses DNA replication, repair, and recombination in microorganisms. The second part is devoted to the interaction of microbes with their environment, which includes stress shock, and the final part of the course offers graduates the latest information on classic exchange mechanisms, such as transformation and conjugation. Through modern approach to the classical topic of microbial genetics, graduates are expected to connect the basic concepts in genetics they got familiar with during their undergraduate studies with the shape and metabolism of microorganisms and to understand specificities of the genetics of microbes as it relates to the genetics of higher organisms. Graduates are also expected to perform independent research during the course and to understand practical implications of the course on their own.

COURSE OBJECTIVE
Introduction to the foundation principles and concepts of modern microbial genetics.

Stimulating active inquiry in a laboratory environment.

Giving an outline of the behavior of genetic material within bacteria and bacteriophage.

Explaining DNA metabolism, genetic response and genetic exchange in connection to microorganisms

COURSE CONTENT
Week
Topic
  1. Prokaryotic DNA replication. DNA repair mechanisms and mutagenesis
  2. Gene expression and its regulation
  3. Bacteriophage genetics. Bacteriophage λ and its relatives
  4. Single-stranded DNA phages
  5. Restriction-modification systems and recombination
  6. Molecular applications
  7. Genetics of quorum sensing circuitry in Pseudomonas aeruginosa
  8. MID-TERM EXAM
  9. Two-component regulation and molecular mechanisms of quorum sensing
  10. Stress shock
  11. Genetic tools for dissecting motility and development of Myxococcus xanthus
  12. Agrobacterium genetics
  13. Bacterial transposons: An increasingly diverse group of elements. Transformation
  14. Conjugation and the subcellular entities (plasmids)
  15. FINAL EXAM

LABORATORY/PRACTICE PLAN
Week
Topic
  1. Prokaryotic DNA replication. DNA repair mechanisms and mutagenesis
  2. Gene expression and its regulation
  3. Bacteriophage genetics. Bacteriophage λ and its
  4. Single-stranded DNA phages
  5. Restriction-modification systems and recombination
  6. Molecular applications
  7. Genetics of quorum sensing circuitry in Pseudomonas aeruginosa
  8. MID-TERM EXAM

  1. Two-component regulation and molecular mechanisms of quorum sensing
  2. Stress shock
  3. Genetic tools for dissecting motility and development of Myxococcus xanthus
  4. Agrobacterium genetics
  5. Bacterial transposons: An increasingly diverse group of elements. Transformation
  6. Conjugation and the subcellular entities (plasmids)
  7. FINAL EXAM

TEACHING/ASSESSMENT
Description
  • Interactive Lectures
  • Practical Sessions
  • Presentation
  • Discussions and group work
Description (%)
Method Quantity Percentage (%)
Midterm Exam(s)130
Presentation130
Final Exam140
Total: 100
Learning outcomes
  • Define gene expression and its regulation
  • Clarify single-stranded DNA phages
  • Describe the genetic tools for dissecting motility and development of Myxococcus xanthus
  • Discuss the molecular mechanism of quorum sensing
  • Explain the transduction in Gram-negative bacteria
TEXTBOOK(S)
  • Willey, J. M. (2008). Prescott, Harley, and Klein\\'s Microbiology-7th international ed. /Joanne M. Willey, Linda M. Sherwood, Christopher J. Woolverton. New York: McGraw-Hill Higher Education.

ECTS (Allocated based on student) WORKLOAD
Activities Quantity Duration (Hour) Total Work Load
Lecture (14 weeks x Lecture hours per week)15345
Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)15230
Midterm Examination (1 week)122
Final Examination(1 week)122
Preparation for Midterm Examination11010
Preparation for Final Examination12020
Assignment / Homework/ Project12121
Seminar / Presentation12020
Total Workload: 150
ECTS Credit (Total workload/25): 6