INTERNATIONAL BURCH UNIVERSITY
Graduate Study - Faculty of Engineering and Natural Sciences
Genetics and Bioengineering Master With Thesis
2014-2015

SYLLABUS
Code Name Level Year Semester
GBE 512 Microbial Genetics Graduate 1 Fall
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
Area Elective 6 3 151 English

Instructor Assistant Coordinator
Mirsada Hukić, Prof. Dr. Mirsada Hukić, Prof. Dr.
[email protected] no email

Please see course objectives below.

COURSE OBJECTIVE
Topics include microbial genomes and their evolution; gene discovery, identification, and mapping; mutation; DNA repair; gene transfer among organisms; plasmids; transposable elements; genetic recombination; and gene regulation. We will also discuss molecular genetic strategies or concepts
including gene cloning, polymerase chain reaction (PCR) and quantitative PCR, hybridization techniques, microarrays, ‘proteomics,’ ‘metabolomics,’ uses of gene expression, directed mutagenesis, gene fusions, ‘reporters,’ probes, and emerging technologies such as ‘Next Generation ’ DNA sequencing strategies.
To introduce the foundation principles and concepts of modern microbial genetics.
To stimulate active inquiry in a laboratory environment

COURSE CONTENT
Week
Topic
  1. Introduction & historical perspective, Prokaryotic genomes
  2. Replication and partitioning of the bacterial genome and Genome of Archaea
  3. Gene Transfer: Impact on microbial evolution & basis for classical mapping and mutation analysis
  4. Conjugation and conjugative plasmid
  5. Transformation: physiological and artificial
  6. Transduction and bacteriophages
  7. Moveable genetic elements
  8. Regulation of gene expression and responses to changing environments
  9. Regulatory RNAs
  10. Bacteriophages and Bacteriophage Genetics
  11. Mutation, DNA repair and evolution
  12. Review of molecular genetics techniques

LABORATORY/PRACTICE PLAN
Week
Topic

    TEACHING/ASSESSMENT
    Description
    • Lectures
    • Excersises
    • Presentation
    • Project
    • Assignments
    Description (%)
    Method Quantity Percentage (%)
    Homework10
    Project20
    Midterm Exam(s)120
    Laboratory10
    Final Exam140
    Total: 100
    Learning outcomes
    • Understanding of principles and concepts of modern and microbial genetics
    • Understanding the role of microbial genetics and molecular biology in the advancement of science and society
    • Reading and evaluating scientific articles
    • Uses of genetic methods to investigate biological problems
    • Exciting current topics in microbial genetics
    TEXTBOOK(S)
    • Microbiology 7th Ed. (2008) Prescott, Harley and Klein
    • http://www.asmusa.org, http://bioweb.uwlax.edu/index.htm, http://www.uwosh.edu/library/, http://www.jgi.doe.gov/JGI_microbial/html/index.html, http://www.expasy.ch/,
    • http://www.ncbi.nlm.nih.gov/, http://www.ncbi.nlm.nih.gov/pubmed/,
    • http://pubchem.ncbi.nlm.nih.gov/, http://www.tigr.org, http://cgsc.biology.yale.edu/,
    • http://www.genome.wisc.edu, http://rebase.neb.com, http://www.rcsb.org/pdb/,
    • http://pymol.sourceforge.net/, http://www.firstmarket.com/cutter/cut2.html,
    • http://www.premierbiosoft.com/netprimer.html, http://www.promega.com/,
    • http://ramsites.net/~biobike/, http://cyanobike, http://www.genome.jp/kegg/,

    ECTS (Allocated based on student) WORKLOAD
    Activities Quantity Duration (Hour) Total Work Load
    Lecture (14 weeks x Lecture hours per week)14228
    Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)14114
    Midterm Examination (1 week)122
    Final Examination(1 week)122
    Preparation for Midterm Examination11515
    Preparation for Final Examination12020
    Assignment / Homework/ Project21530
    Seminar / Presentation22040
    Total Workload: 151
    ECTS Credit (Total workload/25): 6