INTERNATIONAL BURCH UNIVERSITY
Graduate Study - Faculty of Engineering and Natural Sciences
3+2 Genetics and Bioengineering
2014-2015
| SYLLABUS |
| Code |
Name |
Level |
Year |
Semester |
| GBE 512 |
Microbial Genetics |
Graduate |
1 |
Fall |
| Status |
Number of ECTS Credits |
Class Hours Per Week |
Total Hours Per Semester |
Language |
| Area Elective |
6 |
3 |
151 |
English |
| Instructor |
Assistant |
Coordinator |
| Mirsada Hukić, Prof. Dr. |
|
Mirsada Hukić, Prof. Dr. |
| [email protected] |
|
no email |
| Please see course objectives below. |
| COURSE OBJECTIVE |
Topics include microbial genomes and their evolution; gene discovery, identification, and mapping; mutation; DNA repair; gene transfer among organisms; plasmids; transposable elements; genetic recombination; and gene regulation. We will also discuss molecular genetic strategies or concepts
including gene cloning, polymerase chain reaction (PCR) and quantitative PCR, hybridization techniques, microarrays, ‘proteomics,’ ‘metabolomics,’ uses of gene expression, directed mutagenesis, gene fusions, ‘reporters,’ probes, and emerging technologies such as ‘Next Generation ’ DNA sequencing strategies.
To introduce the foundation principles and concepts of modern microbial genetics.
To stimulate active inquiry in a laboratory environment |
| COURSE CONTENT |
- Introduction & historical perspective, Prokaryotic genomes
- Replication and partitioning of the bacterial genome and Genome of Archaea
- Gene Transfer: Impact on microbial evolution & basis for classical mapping and mutation analysis
- Conjugation and conjugative plasmid
- Transformation: physiological and artificial
- Transduction and bacteriophages
- Moveable genetic elements
- Regulation of gene expression and responses to changing environments
- Regulatory RNAs
- Bacteriophages and Bacteriophage Genetics
- Mutation, DNA repair and evolution
- Review of molecular genetics techniques
|
| Description |
- Lectures
- Excersises
- Presentation
- Project
- Assignments
|
| Description (%) |
| Homework | | 10 | | Project | | 20 | | Midterm Exam(s) | 1 | 20 | | Laboratory | | 10 | | Final Exam | 1 | 40 |
|
| Learning outcomes |
- Understanding of principles and concepts of modern and microbial genetics
- Understanding the role of microbial genetics and molecular biology in the advancement of science and society
- Reading and evaluating scientific articles
- Uses of genetic methods to investigate biological problems
- Exciting current topics in microbial genetics
|
| TEXTBOOK(S) |
- Microbiology 7th Ed. (2008) Prescott, Harley and Klein
- http://www.asmusa.org, http://bioweb.uwlax.edu/index.htm, http://www.uwosh.edu/library/, http://www.jgi.doe.gov/JGI_microbial/html/index.html, http://www.expasy.ch/,
- http://www.ncbi.nlm.nih.gov/, http://www.ncbi.nlm.nih.gov/pubmed/,
- http://pubchem.ncbi.nlm.nih.gov/, http://www.tigr.org, http://cgsc.biology.yale.edu/,
- http://www.genome.wisc.edu, http://rebase.neb.com, http://www.rcsb.org/pdb/,
- http://pymol.sourceforge.net/, http://www.firstmarket.com/cutter/cut2.html,
- http://www.premierbiosoft.com/netprimer.html, http://www.promega.com/,
- http://ramsites.net/~biobike/, http://cyanobike, http://www.genome.jp/kegg/,
|
| ECTS (Allocated based on student) WORKLOAD |
| Lecture (14 weeks x Lecture hours per week) | 14 | 2 | 28 | | Laboratory / Practice (14 weeks x Laboratory/Practice hours per week) | 14 | 1 | 14 | | Midterm Examination (1 week) | 1 | 2 | 2 | | Final Examination(1 week) | 1 | 2 | 2 | | Preparation for Midterm Examination | 1 | 15 | 15 | | Preparation for Final Examination | 1 | 20 | 20 | | Assignment / Homework/ Project | 2 | 15 | 30 | | Seminar / Presentation | 2 | 20 | 40 |
|
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