INTERNATIONAL BURCH UNIVERSITY
Graduate Study - Faculty of Engineering and Natural Sciences
Genetics and Bioengineering Master With Thesis
2014-2015

SYLLABUS
Code Name Level Year Semester
GBE 505 Purification Techniques of Biomolecules Graduate 1 Spring
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
6 156 English

Instructor Assistant Coordinator
Yusuf Turan, Prof. Dr. Prof. Dr. Yusuf Turan Yusuf Turan, Prof. Dr.
[email protected] [email protected] no email

The course gives an orientation in the methods that are used for purifying biomolecules. Focus will be on proteins, but other biomolecules will also be treated.The course describes: • Methods to prepare cell extracts and preparation of samples. • Centrifugation, filtering. • Precipitation. • The theoretical and practical bases of chromatography. • Gel-filtration, ion exchange chromatography, hydrophobic interaction chromatography, reverse-phase chromatography, affinity chromatography, etc.

COURSE OBJECTIVE
Purification Techniques of Biomolecules course is designed mainly to accomplish the following objectives;

Explain the intermolecular interactions: mechanism of covalent and noncovalent interactions, charge-charge interactions, hydrogen bonds, Van der Waals forces and hydrophobic interactions.

Describe ionization of water: acid/base chemistry as it applies to biological molecules.

Comprehend structure and function: basic molecular properties of the 4 classes of biological molecules (proteins, carbohydrates, lipids and nucleic acids) and the subunits from which they are formed. Explain how the structure of biological molecules dictates function and how changes in structure direct biochemical reactions.

Define organic reaction mechanisms: the network of chemical reactions that make up central metabolism.

Acquire general background in the field of purification of biomolecules: comprehend scientific literature pertaining to subject matter in purification stratagies.

Approach to scientific mysteries: apply knowledge & concepts to novel problems

COURSE CONTENT
Week
Topic
  1. Introduction; the chemical elements of life, introduction to biomolecules
  2. Matter, elements, atoms, subatomic particles, intermolecular interactions; covalent-iyonik-hydrogen bonds, van der waals interactions
  3. Water and the fitness for biochemical reactions; the pH scale, acid dissociation constant of weak acids, buffered solutions resist changes in pH, low of mass action
  4. Amino acids and primary structures of proteins, some amino acid derivatives and non-protein amino acids
  5. Determination and identification of amino acids; spectrophotometric analysis, ninhydrin reaction mechanism, standard curve for determination
  6. Paper chromatography, thin layer chromatography, high voltage paper electrophoresis, HPLC, active charcoal, PVP
  7. Ammonium sulfate precipitation, dialysis
  8. Midterm
  9. Gel filtration chromatography

  1. Ion exchange chromatography, pI, DEAE, Q-sepharose, CM, SP, FPLC
  2. HIC (Hydrophobic interaction chromatography)
  3. Affinity chromatography
  4. GC (Gas chromatography)
  5. Purification of carbohydrates
  6. Purification of nucleic acids (PAGE)

LABORATORY/PRACTICE PLAN
Week
Topic

    TEACHING/ASSESSMENT
    Description
    • Presentation
    • Assignments
    Description (%)
    Method Quantity Percentage (%)
    Homework1020
    Midterm Exam(s)120
    Presentation120
    Final Exam140
    Total: 100
    Learning outcomes
    • On completion of the course, the students should be able to: Describe and compare different technologies for the purification of biomolecules
    • Plan an efficient purification process for a biomolecule
    • Carry out a chromatographic experiment to purify a biomolecule, calculate yield of the purification, and suggest improvements to reduce losses
    • • Write a scientific report on his experiment
    • Critically review a report and constructively comment on the possibilities for improvements
    TEXTBOOK(S)
    • Reading list will be given each week of the courses. The course book will be complemented with descriptions from other literature and with relevant case studies from research.

    ECTS (Allocated based on student) WORKLOAD
    Activities Quantity Duration (Hour) Total Work Load
    Lecture (14 weeks x Lecture hours per week)14342
    Laboratory / Practice (14 weeks x Laboratory/Practice hours per week) 0
    Midterm Examination (1 week)122
    Final Examination(1 week)122
    Preparation for Midterm Examination12525
    Preparation for Final Examination14040
    Assignment / Homework/ Project14342
    Seminar / Presentation133
    Total Workload: 156
    ECTS Credit (Total workload/25): 6