INTERNATIONAL BURCH UNIVERSITY
Faculty of Engineering and Natural Sciences
Department of Genetics and Bioengineering
2017-2018

SYLLABUS
Code Name Level Year Semester
GBE 326 Cytogenetics Undergraduate 3 Spring
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
5 125 English

Instructor Assistant Coordinator
Lejla Smajlović Skenderagić, Assist. Prof. Dr. Assist. Prof. Dr. Lejla Smajlovic Skenderagic Lejla Smajlović Skenderagić, Assist. Prof. Dr.
[email protected] [email protected] no email

This lecture and laboratory course will focus on human chromosome structure and replication, methodology, form and function, identification and techniques for the visualization of chromosome aberrations. The latter part of the semester focuses on evolution and speciation, sex chromosome systems, artificial manipulation of genomes as well as the human karyotype. Chromosome abnormalities will be discussed from the clinical and cytogenetic viewpoint. The course will also cover current topics in cytogenetics, including new methodologies and their use in clinical genetics and research. Laboratory course covers both experimental techniques in cytogenetics, as well as detailed study of human chromosomes through virtual labs and recitations.

COURSE OBJECTIVE
The cognitive, affective and behavioral objectives of this course are following:
• Introduction to chromosomes, their structure and function.
• Introduction to the cell cycle.
• Preparation of chromosomes for observations.
• Explaining mutations and aberrations.
• Explaining human karyotype.

COURSE CONTENT
Week
Topic
  1. Introduction to cytogenetics
  2. Chromosome structure and replication
  3. Chromosome form and function
  4. Chromosome identification
  5. Nuclear division
  6. Gene control by position and origin
  7. Mutagenesis
  8. MIDTERM EXAM
  9. Chromosome mapping
  10. Sex chromosome systems
  11. Evolution and speciation
  12. Artificial manipulation of genomes
  13. The human karyotype
  14. Chromosomal aberrations and their identification
  15. Historical aspects of cytogenetics

LABORATORY/PRACTICE PLAN
Week
Topic
  1. Beginning of classes
  2. Introduction to lab course
  3. Cell Cycle: Interphase, mitosis, and meiosis
  4. Techniques of making chromosome slides
  5. Chromosome staining techniques, part 1
  6. Chromosome staining techniques, part 2
  7. Getting and marking cells in prophase or prometaphase

  1. MID-TERM EXAM WEEK
  2. Study of sex chromatin
  3. Microscopic observations and microphotography
  4. Nomenclature and chromosome classification
  5. Preparing a karyotype
  6. Common chromosomal abnormalities
  7. Preparation for practical exam
  8. Practical exam from lab course

TEACHING/ASSESSMENT
Description
  • Interactive Lectures
  • Practical Sessions
  • Presentation
  • Discussions and group work
  • Assignments
Description (%)
Method Quantity Percentage (%)
Midterm Exam(s)120
Lab/Practical Exam(s)120
Final Exam140
+Seminar & Assignement 1 20
Total: 100
Learning outcomes
  • Students should be able to describe the cell cycle
  • Students should be able to classify chromosomal banding and its application
  • Students should be able to determine structural and numerical chromosomal aberrations
  • Students should be able to explain the nomenclature of chromosomes
  • Students should be able to analyze karyotipe
TEXTBOOK(S)
  • Clark, M.S., Wall, W.J., (1996) Chromosomes: The complex code, Chapman & Hall

ECTS (Allocated based on student) WORKLOAD
Activities Quantity Duration (Hour) Total Work Load
Lecture (14 weeks x Lecture hours per week)15230
Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)15230
Midterm Examination (1 week)122
Final Examination(1 week)122
Preparation for Midterm Examination11414
Preparation for Final Examination11515
Assignment / Homework/ Project11414
Seminar / Presentation11818
Total Workload: 125
ECTS Credit (Total workload/25): 5