INTERNATIONAL BURCH UNIVERSITY
Faculty of Engineering and Natural Sciences
Department of Genetics and Bioengineering
2016-2017

SYLLABUS
Code Name Level Year Semester
GBE 219 Molecular Biology I Undergraduate 2 Fall
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
Compulsory 5 2 + 2 124 English

Instructor Assistant Coordinator
Monia Avdić - Ibrišimović, Assist. Prof. Dr. Monia Avdić Ibrišimović Monia Avdić - Ibrišimović, Assist. Prof. Dr.
[email protected] [email protected] no email

Molecular Biology I offers students an introduction to the basic principles of DNA, RNA, and proteins, as well as transcription and translation. The course covers the topics on small molecules, macromolecules (structure, shape, and information), energy and biosynthesis, protein function, basic genetic mechanisms, recombinant DNA technology, and control of gene expression. At the end of the course, students are expected to understand the central dogma of molecular biology and to know its specificities in different forms of life: prokaryotes and eukaryotes. This is taken concurrently with a laboratory course, and it is the first part of the two-part molecular biology lecture series.

COURSE OBJECTIVE
The cognitive, affective and behavioral objectives of this course are following:
 Making a detailed study on the structure and function of macromolecules.
 Explaining the nature of the genetic material.
 Giving an overview of the passage of information from gene to protein.
 Explaining the regulation of gene expression.
 Covering techniques of molecular biology.

COURSE CONTENT
Week
Topic
  1. Cells and Genomes
  2. Cell chemistry: The chemical components of the cell
  3. Cell chemistry: How cells obtain energy from food
  4. Shape and structure of proteins
  5. Protein function
  6. Structure and function of DNA, chromosomes and regulation
  7. Midterm week
  8. Global structure of chromosmes and how genomes evolve
  9. Maintenance of DNA replication
  10. DNA repair and chomologous replication, transposition and site specific recombination
  11. From DNA to RNA
  12. From RNA to protein, RNA world
  13. Gene contro, RNA binding motifs
  14. How genetic switches work, specialized cell types
  15. Post transcriptional control

LABORATORY/PRACTICE PLAN
Week
Topic
  1. Beginning of classes
  2. Introductory lab: Short description of lab contents and formation of lab groups
  3. Lab 1: General laboratory rules; Lab equipment; Micropipetting
  4. Lab 2: Intro to spectrophotometer
  5. Lab 3: Isolation of total DNA from banana using commercial detergent
  6. Lab 4: Isolation of total DNA from chicken liver (salting out method)
  7. Lab 5: Determination of DNA concentration and purity by spectrophotometer

  1. MID-TERM EXAM WEEK
  2. Lab 6: Agarose gel electrophoresis with Fast Blast staining
  3. Lab 7: Isolation of human genomic DNA from buccal swab (salting out method)
  4. Lab 8: Introduction to restriction digestion (theory)
  5. Lab 9: Restriction digestion and agarose gel electrophoresis with SafeView Nucleic Acid stain
  6. Lab 10: Restriction mapping (theory)
  7. Preparation for lab exam
  8. Exam from lab course

TEACHING/ASSESSMENT
Description
  • Interactive Lectures
  • Practical Sessions
  • Excersises
  • Presentation
  • Discussions and group work
  • Problem solving
  • Assignments
Description (%)
Method Quantity Percentage (%)
Quiz1212
Problem solving88
Midterm Exam(s)2020
Laboratory1020
Attendance150
Final Exam140
Total: 100
Learning outcomes
  • Recall the molecular structure of DNA/RNA and proteins
  • Describe the basis of the central dogma of molecular biology
  • Explain the molecular mechanisms underlying transcription and translation
  • Illustrate the role of genes and proteins in normal functioning of the cell
  • Examine the basic principles of molecular techniques
  • Conduct isolation of DNA from various material
  • Use spectrophotometry to determine the concentration and purity of DNA/RNA
  • Conduct gel electrophoresis
TEXTBOOK(S)
  • 1. Alberts, B., Johnson, A:, Lewis, J., Morgan, D., Raff, M., Roberts, K., & Walter, P. (2015). Molecular biology of the cell, 6th ed. New York: Garland Science.

ECTS (Allocated based on student) WORKLOAD
Activities Quantity Duration (Hour) Total Work Load
Lecture (14 weeks x Lecture hours per week)28256
Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)14228
Midterm Examination (1 week)122
Final Examination(1 week)122
Preparation for Midterm Examination166
Preparation for Final Examination11414
Assignment / Homework/ Project4416
Seminar / Presentation 0
Total Workload: 124
ECTS Credit (Total workload/25): 5