INTERNATIONAL BURCH UNIVERSITY
Faculty of Engineering and Natural Sciences
Department of Genetics and Bioengineering
2015-2016

SYLLABUS
Code Name Level Year Semester
GBE 219 Molecular Biology I Undergraduate 2 Fall
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
Compulsory 5 2 + 2 817 English

Instructor Assistant Coordinator
Belma Kalamujić, Assist. Prof. Dr. Ms. Adna Ašić Belma Kalamujić, Assist. Prof. Dr.
[email protected] [email protected] no email

The course covers the topics on small molecules, energy, and biosynthesis, macromolecules (structure, shape, and information), how cells are studied, protein function, basic genetic mechanisms, recombinant DNA technology, and control of gene expression.

COURSE OBJECTIVE
The objectives of the course are to make a detailed study on the structure and function of macromolecules, the nature of the genetic material, the passage of information from gene to protein, the regulation of gene expression, the techniques of molecular biology, genomics and bioinformatics.

COURSE CONTENT
Week
Topic
  1. Nucleic acids as genetic information; the “central dogma” of biology
  2. Chemistry and structure of DNA and RNA
  3. Chemical interactions and macromolecular structure
  4. Chromosomes, Chromatin and Nucleosome
  5. DNA replication, Mutability
  6. DNA Reparation Mechanisms
  7. Homologous recombination
  8. Site specific recombination and transposition of DNA
  9. Molecular mechanism of transcription
  10. RNA splicing
  11. Molecular mechanism of translation, the genetic code
  12. Gene regulation in prokaryotes
  13. Gene regulation in eukaryotes
  14. Gene regulation during development
  15. Genome function (including organelar genomes)

LABORATORY/PRACTICE PLAN
Week
Topic
  1. Principles of lab safety
  2. Volume/ weight measurement (micro balance, micro pippetting)
  3. Concentration measurement- Spectrophotometery
  4. Quantitation of nucleic acids and proteins
  5. Reagent preparation. Thereotical and practical discussion
  6. Measurement of pH
  7. Recipes for commonly encountered reagents in molecular biology
  8. Cell culture techniques- Aseptic techniques
  9. Culture of Escherichia coli and related cloning bacteria

  1. Centrifugation technequies
  2. Purification of genomic DNA from wheat and cauliflower
  3. DNA restriction with restriction enzymes and agarose electrophoresis
  4. Protein isolation from wheat and cauliflower
  5. Protein analysis by Bradford method

TEACHING/ASSESSMENT
Description
  • Interactive Lectures
  • Practical Sessions
  • Presentation
  • Assignments
Description (%)
Method Quantity Percentage (%)
Quiz1315
Midterm Exam(s)130
Laboratory1620
Attendance165
Final Exam130
Total: 100
Learning outcomes
  • Actively participate in courses and begin to take responsibility for learning
  • To understand the role of genes and proteins in normal functioning of the cell
  • To learn the basic principles of molecular techniques
  • Begin to work effectively as part of a team, developing interpersonal, organisational and problem-solving skills within a managed environment, exercising some personal responsibility
  • Present information in oral, written, and graphic forms in order to communicate effectively with peers and tutors
TEXTBOOK(S)
  • 1. Alberts, B., Johnson, A:, Lewis, J., Morgan, D., Raff, M., Roberts, K., & Walter, P. (2015). Molecular biology of the cell, 6th ed. New York: Garland Science.
  • 2. Wilson, J. H., & Hunt, T. (2007). Molecular biology of the cell, 5th ed. New York: Garland
  • 3. Watson, J. D., et al (2007). Molecular biology of the gene, 6th ed. Pearson.
  • 4.The condensed Protocols- From Molecular Cloning: A Laboratory Manual,2006, Sambrook, J. and Russell, D.W.

ECTS (Allocated based on student) WORKLOAD
Activities Quantity Duration (Hour) Total Work Load
Lecture (14 weeks x Lecture hours per week)1428392
Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)1428392
Midterm Examination (1 week)122
Final Examination(1 week)122
Preparation for Midterm Examination11414
Preparation for Final Examination11515
Assignment / Homework/ Project 180
Seminar / Presentation 180
Total Workload: 817
ECTS Credit (Total workload/25): 33