INTERNATIONAL BURCH UNIVERSITY
Faculty of Engineering and Natural Sciences
Department of Genetics and Bioengineering
||Number of ECTS Credits
||Class Hours Per Week
||Total Hours Per Semester
||3 + 2
|Enisa Omanović Mikličanin, Prof. Dr.
||Yusuf Turan, Prof. Dr.
|Biochemistry is the field of studies on molecules and chemical reactions of living things. The basic principles of biochemistry are common to all organisms such as bacteria, plants and humans. Therefore any research that is carried out on particular organism, the findings may be applied to almost all other species. The biochemistry course analyzes mainly the structure and function of biomolecules: physical-chemical properties and metabolisms of nucleic acids, proteins, carbohydrates and lipids; the mechanis
|Biochemistry course is designed mainly to accomplish the following objectives;
Understand the energetics of life: the first and second law of thermodynamics to predict the direction of chemical reactions that occur in biological systems.
Explain the intermolecular interactions: mechanism of covalent and noncovalent interactions, charge-charge interactions, hydrogen bonds, Van der Waals forces and hydrophobic interactions.
Describe ionization of water: acid/base chemistry as it applies to biological molecules.
Comprehend structure and function: basic molecular properties of the 4 classes of biological molecules (proteins, carbohydrates, lipids and nucleic acids) and the subunits from which they are formed. Explain how the structure of biological molecules dictates function and how changes in structure direct biochemical reactions.
Describe properties and mechanisms of enzymes: the catalytic functions of enzymes, and perform enzyme kinetics calculations
Define organic reaction mechanisms: the network of chemical reactions that make up central metabolism.
Acquire general background in biochemistry field: comprehend scientific literature pertaining to subject matter in biochemistry.
Approach to scientific mysteries: apply knowledge & concepts to novel problems
- Introduction to biochemistry; the chemical elements of life, intermolecular interactions, introduction to macromolecules, the energetics of life, the cell is the basic unit of life
- Water and the fitness for biochemical reactions; the pH scale, acid dissociation constant of weak acids, buffered solutionsresist changes in pH
- Amino acids and primary structures of proteins; brief information regarding protein purification techniques,basic analytical techniques, protein sequencing strategies
- Three-dimentional structure and function of protein;. Four levels of protein structures,the alpha helix, beta strands and beta sheets, protein folding and stability, structures of miyoglobin and hemoglobin
- Properties of enzymes; the six classes of enzymes, enzyme kinetics, the Michealis-Menten equation, enzyme inhibition, allosteric enzymes, regulation of enzyme activity, mechanisms of enzymes
- Coenzymes and vitamins; coenzyme classification, lipid vitamins, protein coenzymes, cytochromes
- Carbohydrates; Cyclization of aldoses and ketoses, conformations of monosaccharides, disaccharides and other glycosides, polysaccharides, glycoconjugates
- Lipids and membranes; structural and functional diversity of lipids, fatty acids, triacylglycerols, glycerophospholipids, sphingolipids, steroids, biological membrane and lipid bilayers, membrane transport, transduction of intracellular signals
- Introduction to metabolism; major pathways in cells, glycolysis
- Gluconeogenesis, the pentose phosphate pathway, and glycogen metabolism
- The citric acid cycle; conversion of pyruvate to acetyl CoA, the citric acid cycle oxidizes acetyl CoA, the citric acid cycle enzymes, the glyoxilate pathway. Electron transport and ATP synthesis
- Photosynthesis; plant photosynthesis, fixation of CO2 the Calvin cycle, additional carbon-fixation pathways
- Lipid metabolism, amino acid metabolism, nucleotide metabolism
- Nucleic acids, protein synthesis, recombinant DNA technology
- Introductory lab
- Solution preparation, buffers
- Detection of adulteration in milk
- Isoelectric precipitation of proteins
- Quantitative estimation of amino acids by ninhydrin
- Separation of amino acids by TLC
- Titration curves of amino acids
- Construction of maltose standard curve by DNS method
- Qualitative analysis of carbohydrates
- Estimation of saponification value of fats and oils
- Effect of substrate concentration on enzyme kinetics
- Effect of temperature on enzyme kinetics
- Practical Sessions
- Self Evaluation
| Midterm Exam(s)||1||20|
| Final Exam||1||40|
- 1. Moran, Horton, Scrimgeour, & Perry. Principles of Biochemsitry, 5th Edition, Pearson Publishing 2012
- 2. Principles of Biochemistry, Lehninger A., Cox M. 4th Edition, WH Freeman and Co.
- 3. Biochemistry, 5th edition. Jeremy M Berg, John L Tymoczko, and Lubert Stryer
|ECTS (Allocated based on student) WORKLOAD
|Lecture (14 weeks x Lecture hours per week)||14||3||42|
|Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)||14||2||28|
|Midterm Examination (1 week)||1||2||2|
|Final Examination(1 week)||1||2||2|
|Preparation for Midterm Examination||1||15||15|
|Preparation for Final Examination||1||25||25|
|Assignment / Homework/ Project||14||1||14|
|Seminar / Presentation|| || ||0|