INTERNATIONAL BURCH UNIVERSITY
Faculty of Engineering and Natural Sciences
Department of Genetics and Bioengineering
2015-2016

SYLLABUS
Code Name Level Year Semester
GBE 206 Molecular biology II Undergraduate 2 Spring
Status Number of ECTS Credits Class Hours Per Week Total Hours Per Semester Language
Compulsory 5 2 + 2 125 English

Instructor Assistant Coordinator
Belma Kalamujić, Assist. Prof. Dr. Ms. Adna Ašić Belma Kalamujić, Assist. Prof. Dr.
[email protected] [email protected] no email

The second part of a two-part course series in Molecular Biology, the course covers advanced topics on molecules, energy, and biosynthesis. It gives detailed information on all aspects of gene regulation. Additionally, deeper insight into the mechanisms of cell communication and differentiation is given, which includes details of signal transduction. Finally, current techniques in molecular biology are presented, along with model organisms. This is taken concurrently with a laboratory course.

COURSE OBJECTIVE
Students will move beyond their introductory textbooks towards a deeper understanding of modern molecular biology.

COURSE CONTENT
Week
Topic
  1. The cytoskeleton and cell motility
  2. Mechanisms of cell communication
  3. Signal transduction, part I
  4. Signal transduction, part II
  5. Cell differentiation
  6. Epigenetics
  7. RNA polymerase dynamics
  8. Midterm exam
  9. Regulation of gene expression by different types of RNA
  10. RNA silencing pathways
  11. RNA interference
  12. The stability of the genome
  13. Sequence conservation and homology
  14. Techniques in molecular biology, part I
  15. Techniques in molecular biology, part II

LABORATORY/PRACTICE PLAN
Week
Topic
  1. Principles of Lab Safety
  2. Protein isolation from kiwi fruit
  3. Protein isolation from mushroom and ammonium sulfate precipitation
  4. Protein concentration measurement, Bradford method
  5. Protein isolation from chicken liver
  6. Protein isolation from bacterial cell culture
  7. Protein concentration measurement, Lowry method
  8. SDS-PAGE, theory
  9. SDS-PAGE, practice, part I
  10. SDS-PAGE, practice, part II
  11. SDS-PAGE, result interpretation


    TEACHING/ASSESSMENT
    Description
    • Interactive Lectures
    • Excersises
    • Presentation
    • Use of educational films
    Description (%)
    Method Quantity Percentage (%)
    Quiz115
    Midterm Exam(s)130
    Lab/Practical Exam(s)120
    Attendance15
    Final Exam130
    Total: 100
    Learning outcomes
    • Comparitive genomic studies in prokarryotes and eukaryotes
    • Gene expression levels and regulation in prokaryotes and eukaryotes
    • Actively participate in courses and begin to take responsibility for learning
    • Begin to work effectively as part of a team, developing interpersonal, organisational and problem-solving skills with in a managed environment, exercising some personal responsibility
    • Present information in oral, written, and graphic forms in order to communicate effectively with peers and tutors
    TEXTBOOK(S)
    • 1. Alberts, B., Johnson, A., Lewis, J., Morgan, D., Raff, M., Roberts, K., Walter, P. (2015). Molecular Biology of the Cell, 6th ed. Garland Science: New York, NY, USA
    • 2. Karp, G. (2013). Cell and molecular biology: Concepts and experiments, 7th ed. Hoboken, NJ: Wiley

    ECTS (Allocated based on student) WORKLOAD
    Activities Quantity Duration (Hour) Total Work Load
    Lecture (14 weeks x Lecture hours per week)14228
    Laboratory / Practice (14 weeks x Laboratory/Practice hours per week)14228
    Midterm Examination (1 week)122
    Final Examination(1 week)122
    Preparation for Midterm Examination11414
    Preparation for Final Examination11515
    Assignment / Homework/ Project11818
    Seminar / Presentation11818
    Total Workload: 125
    ECTS Credit (Total workload/25): 5